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Fig. 1 | BMC Genomics

Fig. 1

From: Deletion of the fungus specific protein phosphatase Z1 exaggerates the oxidative stress response in Candida albicans

Fig. 1

The physiological effects of CaPPZ1 gene deletion and oxidative stress in C. albicans. The QMY23 control and the cappz1 deletion mutant strains were compared either under normal growth conditions (WT and KO) or after oxidative stress treatment (WTt and KOt). a. The effect of oxidative stress was tested by measuring the optical density at 640 nm before (4 h) and after (5 h) the additions of tBOOH. The mean and SD of 4 independent experiments are shown. b. The colony forming capacity of the same cultures was determined and evaluated as in panel a. c. The effect of tBOOH treatment on the viability of the fungal cells was analyzed by methylene blue. In the representative microscopic images, dead cells are blue and viable cells are unstained. Black bars represent 50 μm. d. The vitality of the C. albicans cells was determined by FungaLight™ staining. The representative fluorescent microscopic images shown vital cells in green and damaged cells in red. White bars represent 25 μm. e. Quantitative analysis of viability (checkered gray bars) and vitality (green bars) tests. The numbers of white and blue objects were counted after methylene blue staining and the intensities of the green and red objects were determined after FungaLight™ staining. The results are expressed as percentages of totals. The mean and SD of three independent experiments are depicted. f. The morphological forms of C. albicans were investigated either under the microscope (checkered bars) or by flow cytometry (clear bars). Shades of turquoise indicate yeast cells and pink show the percentage of hyphae. (Note, that only the percentage of hyphae changed significantly.) The mean and SD of 3 or 4 biological replicates are presented. The significance of the differences was estimated by two-sided, two-sample Student’s t-test and is labelled as * = p < 0.05, ** = p < 0.01, and *** = p < 0.001 in all panels. The experimental data used in this figure can be found in Additional file 3: Dataset S2

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