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Table 1 Comparison of antioxidant enzyme activities and glutathione concentrations of control (WT) and cappz1 deletion mutant (KO) C. albicans strains after 1 h cultivation in the absence or in the presence of 0.4 mM tBOOH (WTt and KOt)

From: Deletion of the fungus specific protein phosphatase Z1 exaggerates the oxidative stress response in Candida albicans

 

WT

WTt

KO

KOt

Catalase [kat (kg protein)−1]

0.5 ± 0.07

0.5 ± 0.09

1.1 ± 0.04 ***,a

2.2 ± 0.15 ***,b; ***,c

Superoxide dismutase [munit (mg protein)− 1]

0.11 ± 0.02

0.11 ± 0.02

0.14 ± 0.02 *,a

0.15 ± 0.02 *,c

Glutathione peroxidase [mkat (kg protein)− 1]

0.3 ± 0.04

0.7 ± 0.2 **,a

0.6 ± 0.1 **,a

0.8 ± 0.1 *,b

Glutathione reductase [mkat (kg protein)− 1]

2.5 ± 0.15

6.9 ± 1.2 ***,a

5.3 ± 0.8 ***,a

9.9 ± 0.8 ***,b; **,c

Reduced glutathione (GSH) [nmol (OD640)− 1]

435 ± 40

548 ± 25**,a

430 ± 35

525 ± 44 *,b

Oxidised glutathione (GSSG) [nmol (OD640)− 1]

3.9 ± 0.4

17.9 ± 2.2 ***,a

8.4 ± 1.7 **,a

20.8 ± 7.2 *,b

GSH/ GSSG

113 ± 17

31 ± 4 ***,a

53 ± 13 **,a

28 ± 11 *,b

  1. All data represent means ± SD calculated from four independent experiments
  2. All of the original assay results used in this table can be found in Additional file 3: Dataset S3
  3. * p < 0.05, ** p < 0.01 and *** p < 0.001 according to Student’s t-test
  4. a Significant difference between the WTt and KO strains in comparison to the untreated WT control strain
  5. b Significant difference between the KOt and untreated KO cells
  6. c Significant difference between the WTt and KOt cells