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Table 2 Tandem repeat analysis in FLO genes. We found seven repeat sequences when analysing flocculation genes FLO1, FLO5, FLO9, FLO10, and FLO11 in S. cerevisiae (S288C) and S. eubayanus (CBS 12357) genomes. These sequences are referred to as sequence A (135 nt), B (15 nt), C (189 nt), D (45 nt), E (132 nt), F (156 nt), and G (30 nt). We used these sequences to analyse the copy numbers of each repeat within all FLO genes in our long-read-only assembly of CBS 1483 using the long-read-only S288C assembly as a control. Their respective copy numbers are shown below. Repeat sequences are indicated in Additional file 1 H

From: Chromosome level assembly and comparative genome analysis confirm lager-brewing yeasts originated from a single hybridization

Species

(Sub)genome

Gene

Gene size (nt)

A

B

C

D

E

F

G

S. cerevisiae

 

S288C

 

FLO1

4614

18.0

9.4

–

–

–

–

–

FLO5

3228

8.0

9.6

–

–

–

–

–

FLO9

3969

13.0

8.3

–

–

–

–

–

FLO10

3510

–

3.8

4.4

–

–

–

–

FLO11

4104

–

–

–

38.7

6.6

–

–

S288C (long)

 

FLO1

4615

18.0

9.4

–

–

–

–

–

FLO5

3228

8.0

9.6

–

–

–

–

–

FLO9

3978

13.0

8.3

–

–

–

–

–

FLO10

3508

–

3.8

4.4

–

–

–

–

FLO11

4104

–

–

–

38.7

6.6

–

–

CBS 1483

 

FLO1 (ScVI)

1038

–

–

–

–

–

–

–

FLO5 (ScI)

2603

1.0

11.1

–

–

–

–

–

FLO9 (ScI)

2967

5.0

15.4

–

–

–

–

–

FLO11 (ScIX)

2787

–

–

–

14.1

5.6

–

–

S. eubayanus

 

CBS 12357

 

FLO1

5517

–

–

–

–

–

24.7

2.8

FLO5

1325

–

–

–

–

–

–

–

FLO9 (SeI)

4752

–

–

–

–

–

8.3

45.9

FLO9 (SeVI)

3480

–

–

–

–

–

–

–

FLO9 (SeX)

4041

–

–

–

–

–

7.4

20.1

FLO9 (SeXII)

3321

–

–

–

–

–

–

10.2

FLO10 (SeXI)

4128

–

–

–

–

–

–

–

FLO11 (SeIX)

4149

–

–

–

–

–

–

–

CBS 1483

 

FLO5 (SeI)

1945

–

–

–

–

–

4.9

2.8

FLO5 (SeI)

391

–

–

–

–

–

–

–

FLO5 (SeVI)

3765

–

–

–

–

–

–

–

FLO5 (SeXI)

2582

–

–

–

–

–

4.9

2.8

FLO9 (SeI)

2100

–

–

–

–

–

3.0

3.8

FLO9 (SeXII)

2892

–

–

–

–

–

–

6.3

FLO10 (SeVI)

3378

–

–

–

–

–

–

–

FLO11 (SeIX)

3909

–

–

–

–

–

–

–