Fig. 2

Differential gene expression in response to M. marinum and L. pneumophila infection. a–c Summary of gene regulation in D. discoideum in response to separate infections with M. marinum a and L. pneumophila 1 hpi and 6 hpi b, c, respectively. X-axes represent number of genes (FDR < 0.05) and Y-axes display the regulation of genes in comparison to non-infected controls. d RT-qPCR validation of differential expression of genes in response to M. marinum infection. RT-qPCR was performed on RNA from the same two infection experiments used for RNA-seq, including respective non-infected controls for differential expression analyses. e Comparison of gene regulation detected by microarray [23] with the corresponding regulation determined with RNA-seq for L. pneumophila infected cells. Marked in blue: genes with log2(fold change) bigger than 1 or smaller than − 1 according to both methods; Marked in black: genes with log2(fold change) bigger than 1 or smaller than − 1 according to microarray but not RNA-seq; Marked in green: genes showing opposite regulation according to the different methods. Note that the ranges for the two axes differ