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Fig. 1 | BMC Genomics

Fig. 1

From: Trans-splicing of mRNAs links gene transcription to translational control regulated by mTOR

Fig. 1

The mTOR-regulated translatome of O. dioica. a Adult animals were exposed to the mTOR inhibitor, Torin 1 (1 μM), or DMSO (vehicle control) in seawater for 1.5 h and female animals were collected. Phosphorylated 4E-BP1 was detected in DMSO but not in Torin 1 treated animals, confirming that mTOR was inhibited in Torin 1-treated animals (one out of three replicates shown; total protein used as a loading control and normalisation of band intensity; see Additional file 1: Figure S1D for full blot). b Median translational efficiency (RPF/mRNA = ribosome protected fragment density/mRNA density) of mRNAs from 3 replicates for Torin 1- and DMSO-treated animals with transcripts identified as having significantly up- or down-regulated translation highlighted. c Translational efficiencies shown as in (b) with known Torin 1-resistant (histone mRNAs) and Torin 1-sensitive (ribosomal protein mRNAs) gene categories highlighted to show that targets of mTOR-mediated translational control are conserved in O. dioica. d Intersections of orthologs of known TOP mRNAs (TOP), mTOR-regulated mRNAs (mTOR) and mRNAs that are trans-spliced (SL)

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