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Fig. 3 | BMC Genomics

Fig. 3

From: Trans-splicing of mRNAs links gene transcription to translational control regulated by mTOR

Fig. 3

Abundant maternal mRNAs stocked in the oocyte are trans-spliced, translationally dormant and resistant to mTOR-inhibition. a Distribution of translational efficiencies (ribosome density normalised to mRNA abundance; RPF = ribosome protected fragments) in control (DMSO) animals with transcripts categorised according to the presence of the 5′ spliced leader and their response to mTOR inhibition (suppressed or unaffected). b Distribution of mRNA abundances (RPM = reads per million) in control (DMSO) animals with transcripts categorised as in the colour legends under a and b. c Distribution of translational efficiencies in control animals with transcripts categorised by the presence of the 5′ spliced leader and whether or not they are present in oocytes (detected by cap analysis of gene expression (CAGE) or tiling microarray from oocyte samples). d The cytoplasm of the coenocyst in day 5 (D5) female gonads, pre-oocyte formation, has a high level of translational activity as indicated by the intensity of green Alexa Fluor® 488 detecting homopropargylglycine (HPG), an amino acid analog that is incorporated during protein synthesis. Nurse nuclei (NN) and meiotic nuclei (MN) are also visible. Oocytes (Oo) that have formed by late day 6 (D6), however, are translationally dormant. Red Alexa Fluor® 568-labelled staining shows the location of mRNAs that have a 5′ trimethylguanosine (TMG) cap, which is present on the spliced leader. DNA was counterstained with blue To-Pro-3 iodide (e) RNA from D5 animals have higher levels of polysome occupancy compared to RNA from oocytes, where there are no visible polysome peaks

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