Fig. 2

Molecular phylogeny of newly sequenced strains. (a) Phylogenetic tree of 496 publicly available Streptomyces genomes. Mycobacterium tuberculosis H37Rv was used as outgroup. Select regions of the atpD, gyrB, recA, rpoB, and trpB genes were concatenated and used to generate a multi-locus alignment in the MEGA7 software package. Genetic distances (average nucleotide identity) generated from the multisequence alignment were used to build a phylogenetic tree using the maximum likelihood method. Clades containing the newly sequenced genomes are S. katrae (S3–4, blue), S. virginiae (3211–3, green), and S. lydicus (GS93–23, red). Subtrees composed of S. katrae and S. virginiae (b), and S. lydicus (c) showing the newly sequenced isolates and their closest relatives