Skip to main content
Fig. 1 | BMC Genomics

Fig. 1

From: Efficient depletion of ribosomal RNA for RNA sequencing in planarians

Fig. 1

Efficiency of rRNA removal from total planarian RNA. a Schematic representation of rRNA depletion workflow. Biotinylated DNA probes are hybridized to rRNA, followed by subtraction of DNA-rRNA hybrids using streptavidin-coated magnetic beads. b Separation profile of planarian total RNA. The large peak at 1527 nts corresponds to the co-migrating 18S rRNAs and the two fragments of processed 28S rRNA. LM denotes the lower size marker with a length of 15 nts. c Increasing concentration of NaCl improves the efficiency of rRNA removal. d Total planarian RNA after rRNA depletion. e Removal of DNA-rRNA hybrids was performed in two consecutive steps using streptavidin-coated magnetic beads resuspended in 2x of 1x B&W buffer

Back to article page