Table 1 Assessment of phenotypic traits related to boar sperm DNA integrity. Data (n = 18) related to sperm DNA integrity parameters on the day of semen collection (Day 0) and upon liquid preservation at 18 °C for 96–108 h (Day 4), shown as mean ± SEM. For DFI at Day 0 (n = 13)
From: DNA methylation patterns vary in boar sperm cells with different levels of DNA fragmentation
Day 0 | Day 4 | ||
|---|---|---|---|
Free thiols (mFI) | min – max | 4848.0–12,295.3 | 4401.3–13,353.0 |
mean ± SEM | 7133.7 ± 557.8 | 7489.5 ± 657.2 | |
Total thiols (mFI) | min – max | 42,778.6–48,306.3 | 33,912.7–45,298.0 |
mean ± SEM | 44,850.7 ± 374.3 | 41,477.0 ± 705.1 *** | |
Disulphide bonds (mFI) | min – max | 16,841.8–21,729.1 | 11,656.3–20,143.1 |
mean ± SEM | 18,854.5 ± 263.6 | 16,993.7 ± 501.7*** | |
Protamine deficiency (mFI) | min – max | 2386.1–3719.4 | 2608.5–4756.0 |
mean ± SEM | 2915.9 ± 94.8 | 3553 ± 164.4 *** | |
DFI (%) | min – max | 0.3–20.4 | 0.4–27.4 |
mean ± SEM | 6.0 ± 1.6 | 7.8 ± 1.9 *** |