Fig. 2

Annotation, expression profiles and prediction comparison for miR-499.We annotated the pre-miR-499 on sgr09, position 55,926,017– 55,926,134 and the two mature miRNAs at 55,926,048–55,926,069 and 55,926,085–55,926,106. The six methods used for miRNA detection are displayed, CID-miRNA was not able to detect this miRNA. Tools working independent of the small RNA-Seq data BLAST (cyan), Infernal (olive green) and goRAP (orange) vary in their annotation length. The latter two programs are based on covariance models, identifying mostly the complete pre-miRNA. The remaining two programs miRDeep* and Blockbuster are based on small RNA-Seq data (*) and therefore accurately annotate the mature miRNAs. MiR-499 is expressed weakly within N. furzeri MZM 12 month liver library and therefore could not be detected by miRDeep* and Blockbuster. In the N. furzeri MZM 12 month brain library, miR-499 was expressed strongs enough to be detected by both programs