Transcriptome analysis reveals mechanism underlying the differential intestinal functionality of laying hens in the late phase and peak phase of production

Table 3 Characteristics¹ of RNA sequencing reads of the layer intestine (n = 4)

Samples²	GC contents (%)	Q30 (%)	Total reads	Mapped reads	Mapping ratio	Unique mapping ratio
PP1	50.67	92.88	58,014,476	52,888,432	91.16%	87.47%
PP2	50.06	92.49	50,793,752	46,281,638	91.12%	87.63%
PP 3	50.37	92.89	56,232,772	50,630,631	90.04%	86..52%
PP4	50.87	93.19	46,453,748	36,633,802	78.86%	75.66%
LP1	49.85	92.35	49,218,916	44,799,521	91.02%	87.79%
LP2	49.94	93.40	63,324,840	58,066,099	91.70%	88.36%
LP3	49.28	92.04	40,910,976	36,615,172	89.50%	86.26%
LP4	50.16	93.35	43,921,962	40,084,927	91.26%	87.70%

¹GC guanine-cytosine, Q30 the proportion of bases with a Phred quality score greater than 30
²PP laying hens in the peak phase of production, LP laying hens in the late phase of production

ISSN: 1471-2164