Fig. 1

Morphology and growth of alkaligrass calli under NaCl stress. a-f Morphology of the callus cultured on MS medium. The 45-day-old callus was transferred to MS medium supplemented with 0, 50, and 150 mM NaCl (a-c), and was cultured for additional 28 days (d-f). Bar = 1.5 cm. g Callus relative growth rate (n = 20). h Callus cell viability (n = 8). i Malondialdehyde content (n = 3). Values are presented as means ± standard deviation. The values were determined from callus under 0, 50, and 150 mM for 28 days. Significant differences between control and treatments are marked with asterisks (* represents p < 0.05, ** represents p < 0.01)