Fig. 5

Abundance patterns and subcellular localization of NaCl-responsive proteins in the alkaligrass calli. a Overlap between protein lists from three comparison of NaCl treatments. The outer circle represents three comparisons of NaCl treatment conditions (i.e., 50 mM vs 0 mM, 150 mM vs 0 mM, and 150 mM vs 50 mM). The inner circle represents NaCl-responsive protein lists of each treatment conditions, where hits are arranged along the arc. Proteins that hit multiple lists are colored in dark orange, and proteins unique to a list are shown in light orange. The purple and blue curves link the same proteins among three lists. b Graphical of the numbers of proteins in various functional categories. The numbers in the y axis are referred in names of function categories in (d). c Subcellular localization analysis of 55 NaCl-responsive proteins. Subcellular localization categories of proteins predicted by internet tools and knowledge from literatures. The numbers of proteins with different locations are shown in the pie. d Abundance patterns of NaCl-responsive proteins in the alkaligrass calli. Three columns represent different treatments of 0, 50, and 150 mM NaCl. The rows represent individual proteins. The increased or decreased proteins are indicated in red or blue, respectively. The color intensity increases with increasing abundant differences, as shown in the scale bar. The scale bar indicates relative protein abundance ratios ranging from 0.1 to 6. The abbreviations of protein name are listed on the right side, and the full names are listed in Additonal file 2: Table S1. The protein names marked with a pentagram (*) were edited according to the functional domain annotations from NCBInr protein database. Abbreviation: 14–3-3¹, 14–3-3-like protein A; 14–3-3², 14–3-3-like protein GF14–12 isoform X1; α-Gal, Alpha-galactosidase; ACT7, Actin 7 isoform 1; ACY, Aminoacylase-1; AGPR, N-acetyl-gamma-glutamyl-phosphate reductase; AKR2, Aldo-keto reductase 2; APX, Ascorbate peroxidase; BADH, Betaine aldehyde dehydrogenase; CAL1, Carbonic anhydrase-like1; CPO, Oxygen-dependent coproporphyrinogen-III oxidase; Csase, Cysteine synthase; EF2, Elongation factor2; ENO, Enolase; Fer, Ferritin; FQR1, NAD(P)H dehydrogenase (quinone) FQR1-like; G6PDH, 6-phosphogluconate dehydrogenase 1; GAPDH2, Glyceraldehyde-3-phosphate dehydrogenase 2; GSTI, Glutathione S-transferase I; HSP70, Heat shock 70 kDa protein; HSP90, Cytosolic heat shock protein 90.1; HOP, Hsp70-Hsp90 organizing protein 1; IDH, Isocitrate dehydrogenase; Macro, Macro domain-containing protein VPA0103; mATPS, ATP synthase; SIR, Sulfite reductase; MDH, Malate dehydrogenase; MDHAR, Monodehydroascorbate reductase like; PDC, Pyruvate decarboxylase; POD12, Peroxidase12; PPP, Pentose phosphate pathway; RAD23, DNA repair protein RAD23; RH2, DEAD-box ATP-dependent RNA helicase 2; SAHH, S-adenosyl-L-homocysteine hydrolase; STOI, Salt tolerance protein I; SUS, Sucrose synthase; TCP1, T-complex protein 1 subunit theta; TSJT1, Stem-specific protein TSJT 1 like