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Table 7 Runtime comparison for transcript assembly of single cell dataset with and without the Falco framework

From: Cloud accelerated alignment and assembly of full-length single-cell RNA-seq data using Falco

System

Nodes

Human - brain (hours)

  

STAR + StringTie (with reference)

STAR + Scallop

HISAT + StringTie

Standalone

1 (1 process)

17.2

16.2

16.3

 

1 (12 processes)

4.2

5.5

5.7

 

1 (16 processes)

N/A

N/A

4.1

Falco

10 node

2.9

2.8

2.3

 

20 node

1.7

1.7

1.4

 

30 node

1.3

1.3

1.1

 

40 node

1.1

1.1

0.9

  1. Standalone number of processes indicates the number of FASTQ file pairs that are processed in parallel. Timing for Falco includes initialisation and configuration time which are approximately 10 min. Runtime for STAR with 16 processes is not available as some STAR processes are killed by the operating system, resulting in failure of the job