Fig. 4

Immune infiltration in syngeneic models. a Gene expression of immune cell type, immune cell activation and immune suppression markers in cells grown in vitro and tumor tissues from the transplantation. Gene expression shown as log2 of transcript per million (TPM) and standardized across samples. b Unsupervised clustering analysis of immune marker expression in tumor tissues from the transplantation separates syngeneic models into high and low infiltration models. c Comparison of cytolytic activity of solid tumor syngeneic models with tissue matched human tumors from TCGA (human data were downloaded from Rooney et al. [17]).Cytolytic activity (CYT) is defined as the log-average (geometric mean) of Gzma and Prf1 expression in transcripts per million (TPM) as describe by Rooney et al.