Table 3 Sequences of the primers used in this study
| Gene Id | Protein | Function | Primer sequence |
|---|---|---|---|
| Peroxidasin | Peroxidase | antimicrobial defense |
F: TAGAAACAGCGTCCTCAACAGTTAG R: ATCATAATCCGGTGTTAAAG |
| GST2 | Glutathione S-transferase | detoxification (oxidative stress reponse) |
F: TTCATCTTCACCTTCCGAACTAAAG R: TGCCAGTGCTTGAAATAACCGAC |
| C1q2 | Complement like factors | Pathogen recognition |
F: TTAGCAATATCATACGGGATAG R: AGAACAGCTGTAAATGCGATGACAT |
| C1qB | Complement like factors | Pathogen recognition |
F: TTAATACAAATGTTGTTGCCGACAC R: TGTGGCCATTGAATGCTTATTGC |
| C1q3 | Complement like factors | Pathogen recognition |
F: GCGTTTTGGTGACAATTACATGTTC R: TTGGTCAAATTTTATTACTAAGCC |
| SOCS | Suppressor of cytokine signaling | signal transduction |
F: AAACCGACGGTAACGAGAAT R: TAAATATTTAGAATCCGAACTATCA |
| STAT | Signal transducers and activators of transcription | signal transduction |
F: ATCCCGTATTTCTGCTCGGC R: GTGCGATGGCTTGTTCATGG |
| Perlucin | Shell matrix protein/defense molecule | cellular component/ immunity |
F: TCTACGTTTGGCTGAAGTCGGTCTA R: GATGGCCCTTATATGTCAAT |
| MR2 | Macrophage receptor with collagenous structure | Scavenging (innate immunity) |
F: CAGGCAAGTGTTTTCTCGTGTTGGC R: GTCCGGCAAGGTAGTAGCTT |
| BGRP | Beta-glucan recognition protein | Pattern recognition (innate immunity) |
F: AACGGCATATCTTTAGTAGCAT R: TGAGGTTGTTGGACTAGACGCTGTC |
| Caspase 3 | Protease | Apoptosis |
F: CCTCCAGAACCAAGAAGCGT R: CTGGGGTTAAGATGCCACGT |
| IAP | inhibitor of apoptosis | balance between cell proliferation and cell death by inhibiting caspase activity and facilitating immune responses |
F: TATGGTAAAATGGAAGACGC R: CCACCACTGCTTCTTTGTCTAAACA |
| FADD | Fas-Associated protein with Death Domain | apoptosis |
F: GGTACACCAAGCTCTCGCAT R: TGAGAGGACATGTCGAGGCT |
| TNF | Tumor necrosis factor | immunity |
F: TGGTTGTTCTGCATTCGCTTGTTAC R: AATGTTCAGAAATCGGAATTGGT |
| Tubulin | centrosomal protein | Cell division; oxidative stress |
F: AGAGACTGGAGCTGGCAAACACGTA R: GAGCATAGTTGTTGGCAGCGTC |
| Calreticulin | Endoplasmic reticulum chaperon | Calcium homeostasis and protein maturation; oxidative stress |
F: AGATATGTACGGAGAATCACCTTAC R: TACCACTTTCTACTTTAGCGTT |
| Rho-J | GTP-binding protein | Signal transduction |
F: GAAGGACTGCGCGTGTTTACTTAC R: TTGCTCCAATCTTATTCGCCAATTT |
| Rab-5C | Ras-related protein | Endocytosis |
F: GCCGACTGAGGTCTTAACTT R: ACAAGCAGCCGTCGTAGTATATGAC |
| CYTB | cytochrome b oxidase | Oxidative phosphorylation |
F: ACAAGACTCCGGCGCATATT R: AAAGGTCTTTCCACAGGGCAAGTCC |
| COX3 | cytochrome c oxidase subunit 3 | Oxidative phosphorylation |
F: CTGCAGTATTCGGAGTATAAGTGGT R: AACGACGGAATGCGAAGTGAT |
| COX1 | cytochrome c oxidase subunit 1 | Oxidative phosphorylation |
F: GTTACTGCTCATGGGCTAGTG R: ATCCTCAACCCAAACAGACCTTAAT |
| ND5 | NADH dehydrogenase subunit 5 | Oxidative phosphorylation |
F: GGGGGTATATGTATTACTTC R: CTCCCAATCAAAAACACTATAATCC |
| ND1 | NADH dehydrogenase subunit 1 | Oxidative phosphorylation |
F: CCCCGCCCCGTATTCTACAT R: ATAAGGGTTATTATTTGGGCAGGC |