Skip to main content

Table 3 Sequences of the primers used in this study

From: Comparative transcriptomic analysis of surf clams (Paphia undulate) infected with two strains of Vibrio spp. reveals the identity of key immune genes involved in host defense

Gene Id

Protein

Function

Primer sequence

Peroxidasin

Peroxidase

antimicrobial defense

F: TAGAAACAGCGTCCTCAACAGTTAG

R: ATCATAATCCGGTGTTAAAG

GST2

Glutathione S-transferase

detoxification (oxidative stress reponse)

F: TTCATCTTCACCTTCCGAACTAAAG

R: TGCCAGTGCTTGAAATAACCGAC

C1q2

Complement like factors

Pathogen recognition

F: TTAGCAATATCATACGGGATAG

R: AGAACAGCTGTAAATGCGATGACAT

C1qB

Complement like factors

Pathogen recognition

F: TTAATACAAATGTTGTTGCCGACAC

R: TGTGGCCATTGAATGCTTATTGC

C1q3

Complement like factors

Pathogen recognition

F: GCGTTTTGGTGACAATTACATGTTC

R: TTGGTCAAATTTTATTACTAAGCC

SOCS

Suppressor of cytokine signaling

signal transduction

F: AAACCGACGGTAACGAGAAT

R: TAAATATTTAGAATCCGAACTATCA

STAT

Signal transducers and activators of transcription

signal transduction

F: ATCCCGTATTTCTGCTCGGC

R: GTGCGATGGCTTGTTCATGG

Perlucin

Shell matrix protein/defense molecule

cellular component/ immunity

F: TCTACGTTTGGCTGAAGTCGGTCTA

R: GATGGCCCTTATATGTCAAT

MR2

Macrophage receptor with collagenous structure

Scavenging (innate immunity)

F: CAGGCAAGTGTTTTCTCGTGTTGGC

R: GTCCGGCAAGGTAGTAGCTT

BGRP

Beta-glucan recognition protein

Pattern recognition (innate immunity)

F: AACGGCATATCTTTAGTAGCAT

R: TGAGGTTGTTGGACTAGACGCTGTC

Caspase 3

Protease

Apoptosis

F: CCTCCAGAACCAAGAAGCGT

R: CTGGGGTTAAGATGCCACGT

IAP

inhibitor of apoptosis

balance between cell proliferation and cell death by inhibiting caspase activity and facilitating immune responses

F: TATGGTAAAATGGAAGACGC

R: CCACCACTGCTTCTTTGTCTAAACA

FADD

Fas-Associated protein with Death Domain

apoptosis

F: GGTACACCAAGCTCTCGCAT

R: TGAGAGGACATGTCGAGGCT

TNF

Tumor necrosis factor

immunity

F: TGGTTGTTCTGCATTCGCTTGTTAC

R: AATGTTCAGAAATCGGAATTGGT

Tubulin

centrosomal protein

Cell division; oxidative stress

F: AGAGACTGGAGCTGGCAAACACGTA

R: GAGCATAGTTGTTGGCAGCGTC

Calreticulin

Endoplasmic reticulum chaperon

Calcium homeostasis and protein maturation; oxidative stress

F: AGATATGTACGGAGAATCACCTTAC

R: TACCACTTTCTACTTTAGCGTT

Rho-J

GTP-binding protein

Signal transduction

F: GAAGGACTGCGCGTGTTTACTTAC

R: TTGCTCCAATCTTATTCGCCAATTT

Rab-5C

Ras-related protein

Endocytosis

F: GCCGACTGAGGTCTTAACTT

R: ACAAGCAGCCGTCGTAGTATATGAC

CYTB

cytochrome b oxidase

Oxidative phosphorylation

F: ACAAGACTCCGGCGCATATT

R: AAAGGTCTTTCCACAGGGCAAGTCC

COX3

cytochrome c oxidase subunit 3

Oxidative phosphorylation

F: CTGCAGTATTCGGAGTATAAGTGGT

R: AACGACGGAATGCGAAGTGAT

COX1

cytochrome c oxidase subunit 1

Oxidative phosphorylation

F: GTTACTGCTCATGGGCTAGTG

R: ATCCTCAACCCAAACAGACCTTAAT

ND5

NADH dehydrogenase subunit 5

Oxidative phosphorylation

F: GGGGGTATATGTATTACTTC

R: CTCCCAATCAAAAACACTATAATCC

ND1

NADH dehydrogenase subunit 1

Oxidative phosphorylation

F: CCCCGCCCCGTATTCTACAT

R: ATAAGGGTTATTATTTGGGCAGGC