Table 4 Melanin Biosynthesis Genes

PKS gene cluster

Query sequences

DHN melanin biosynthesis

(A. fumigatus Af293)

DHN melanin biosynthesis (Botrytis cinerea)

alb1(PKS)

g8340.t1 (99%)

g5144.t1 (60% id with Bcpks12, 46% id with Bcpks13)

g6238.t1 (49% id with Bcpks12 and Bcpks13)

g7880.t1 (54% id with Bpks12)

g4227.t1 (49% id with Bpks12 and Bpks13, 45% id with alb1)

arp1(scytalone reductase)

g8339.t1 (100%)

g6728.t1 (51% id with Bcscd1 and Bcscd2, 58% id with arp1)

g718.t1 (51% id with arp1, 48% id with Bscd1)

g9110.t1 (54% id with Bcscd1, 58% id with arp1)

g4314.t1 (48% id with Bcscd1, 53% id with arp1)

arp2 (hydroxynaphthalene reductase)

g8338.t1

(100%)

g3356.t1 (55% id with B. cinerea Bcbrn1)

g9077.t1 (59% id with Bcbrn1 Condensin complex subunit 2)

g2251.t1 (78% id with Bcbrn1)

g5146.t1 (61% id with Bcbrn2 SDR)

g2727.t1 (52% id with arp2)

g2550.t1 (54% id with Bcbrn1)

g6236.t1 (69% id with Bcbrn1)

g2727.t1 (59% id with Bcbrn2)

g10828.t1 (59% id with Bcbrn1)

g11454.t1 (79% id with Bcbrn1)

g11454.t1 (50% id with arp2)

g5031.t1 (58% id with Bcbrn2)

g2131.t1 (53% id with arp2)

g1699.t1 (52% id with Bcbrn1)

g4229.t1 (70% id with Bcbrn1)

g2131.t1 (60% id with Bcbrn2)

ayg1 (conidial pigment biosynthesis protein yellowish-green1)

g8337.t1

(99%)

Bcygh1 (Abhydrolase)

g5555.t1 (64%)

g10909.t1 (50% id with A. fumigatus ayg1)

g10909.t1 (54% id with Bcygh1)

g4919.t1 (60% id with Bcygh1)

g4919.t1 (50% id with ayg1)

g8790.t1 (49% id with Bcygh1)

g8790.t1 (51% id with ayg1)

abr1 (conidial pigment biosynthesis oxidase Abr1/brown 1)

g8336.t1 fragment (100% id last half of query)

g8335.t1

(99%)

<  50% id

<  50% id

<  50% id

<  50% id

abr2 (conidial pigment biosynthesis oxidase/laccase)

g8334.t1 (99%)

<  50% id

<  50% id

<  50% id

<  50% id

Genome

FGC_1

Aspergillus CK392

FGC_2

Coniochaeta CK134

FGC_3

Embellisia CK46

FGC_4

Chaetomium CK152

FGC_5

Phoma CK108

Gene

Eumelanin biosynthesis

Tyrosinase

1.14.18.1

g1264.t1

g1168.t1

g4438.t1

g6854.t1

g157.t1

g9197.t1

g5603.t1 g9014.t1 g9677.t1 g9249.t1

g145.t1

g784.t1

g2521.t1 g4858.t1 g2380.t1 g1694.t1

Genome

FGC_1

Aspergillus CK392

FGC_2

Coniochaeta CK134

FGC_3

Embellisia CK46

FGC_4

Chaetomium CK152

FGC_5

Phoma CK108

Gene

Pyomelanin biosynthesis

Tyrosine aminotransferase tat/ aromatic aminotransferase

2.6.1.5/2.6.1.57

g5120.t1

g776.t1

(100% id, Aspergillus fumigatus Af293)

g2830.t1

g4574.t1

(84% id, Coniochaeta ligniaria NRRL 30616)

g10171.t1

g2384.t1

(92% id, Alternaria alternata)

g10445.t1

(95% id, Colletotrichum salici

g8502.t1

(90% id, Epicoccum nigrum)

4-hydroxyphenylpyruvate dioxygenase

hppD

1.13.11.27

g986.t1

g4873.t1

g885.t1

g8201.t1

g6853.t1

g2062.t1

(Aspergillus fumigatus Af293)

g3403.t1

(80% id,

Coniochaeta ligniaria NRRL 30616)

g4879.t1

(92% id, Coniochaeta ligniaria NRRL 30616)

g5965.t1

(84% id, Alternaria alternata)g9210.t1(96% id, Alternaria alternata)

g935.t1

(80% id, Coniochaeta ligniaria NRRL 30616 g3716.t1

(87% id, Thermothelomyces thermophila ATCC 42464)

g2423.t1

(73% id, Alternaria alternata)

g7746.t1

(93% id, Ascochyta rabiei)

auto-oxidation (spontaneous oxidation in air)

step followed by polymerization into pyomelanin

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+

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+

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