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Fig. 5 | BMC Genomics

Fig. 5

From: A phenomics-based approach for the detection and interpretation of shared genetic influences on 29 biochemical indices in southern Chinese men

Fig. 5

The impact of ALDH2 rs671 on osteogenic and adipogenic differentiation of 3 T3-L1 preadipocytes. a The cell growth curve measured as 450 nm absorbance by using Cell Counting Kit-8 Annexin V-FITC/PI–labelled cells was detected by flow cytometry to measure osteoblast apoptosis. Representative dot plots b and quantified data as the percentage of total cells c At 7 days after osteoblast induction, cells were stained with Alizarin Red S solution to measure calcium content. Representative photographs d and quantified Alizarin red S staining in cells. e Expression of osteocalcin-related genes (AKP, osteocalcin, Runx2, Col1) in ALDH2 WT- or Glu504Lys-overexpressing 3 T3-L1 preadipocytes after 7 days of induction refer to 3 T3-L1 RFP. f At 7 days after adipocyte induction, cells were stained with Oil Red O to measure triglyceride (TG) content. Representative photographs g and quantified Oil Red O staining in cells. h qPCR analysis of adipogenic (adiponectin, C/EBPα, C/EBPβ, Fabp4, Pparγ) expression in ALDH2 WT- or Glu504Lys-overexpressing 3 T3-L1 preadipocytes after 7 days of induction refer to 3 T3-L1 RFP. i Data are shown as the mean ± SE from 3 independent experiments. * P < 0.05, **P < 0.01; ***P < 0.001

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