Fig. 1

The number of genes differentially regulated in the WT and Sphk2−/− mice exposed to PA shown as a Venn diagram. Data was analyzed using two-way ANOVA. Two-way ANOVA represents the analyses performed to ascertain the impact of three factors such as 1. Sphk2 gene KO, 2.PA infection, 3. Interaction of the gene KO and infection There are three circles representing the data derived from the two-way ANOVA. Circle labelled genotype shows genes affected by knockout of Sphk2 gene. Sphk2^−/− shows only 2 genes differentially regulated when the corresponding gene was knocked out. Circle labelled infection shows genes affected by PA. 375 genes differentially regulated by PA group compared to the corresponding control unaffected by other factors. The third circle shows genes affected by the interaction between the two factors ie Sphk2^−/− and PA. 1496 genes were solely affected by the interaction between Sphk2^−/− and PA