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Fig. 12 | BMC Genomics

Fig. 12

From: Genetic deletion of Sphk2 confers protection against Pseudomonas aeruginosa mediated differential expression of genes related to virulent infection and inflammation in mouse lung

Fig. 12

Validation by Western Blot of differentially regulated genes in microarray. WT or Sphk2−/− mice were treated with Pseudomonas aeruginosa (PA) or vehicle (Veh) for 24 h following which the mice were euthanized, lungs removed for protein and RNA extraction as described in Materials and Methods. Whole lung homogenates were subjected to SDS-PAGE and Western blotting . Immunoblot showed increased expression of JmjD3, Spata 13 and ODC1 in the PA exposed lungs of the WT mice compared to Sphk2−/− mice. Clec1A expression was significantly decreased in Sphk2−/− exposed to PA, compared to WT PA infected mouse lung. Western blots probed with appropriate primary and secondary antibodies were quantified by densitometry and normalized to the corresponding total protein

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