Fig. 4

Analysis and validation of differentially expressed circular RNA (circRNA). a The heat map of the circRNA that were differentially expressed between the thermoneutral (TN) group and the heat stress (HS) group. Blue represents low expression, and orange represents high expression (P < 0.05). b Expression of differentially expressed circRNA was determined with data from RNA sequencing (RNA-seq; 3 samples each group). c Expression of differentially expressed circRNA was determined using quantitative real-time PCR (qRT-PCR; 6 samples each group). Values are expressed as means and SEM. *P < 0.05; **P < 0.01 (t-test). HS_P1 = heat stress_pituitary 1; HS_P2 = heat stress_pituitary 2; HS_P3 = heat stress_pituitary 3; TN_P1 = thermoneutral _pituitary 1; TN_P2 = thermoneutral _pituitary 2; TN_P3 = thermoneutral _pituitary 3