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Fig. 3 | BMC Genomics

Fig. 3

From: Genome sequence of Pseudomonas aeruginosa PAO1161, a PAO1 derivative with the ICEPae1161 integrative and conjugative element

Fig. 3

Influence of mutations in PA2735 or PA1939 on plasmid transformation of P. aeruginosa cells. a Transformation frequency of P. aeruginosa strains transformed with plasmids. pCM132 containing 3 sequence motifs recognized by PA2735 and pOMB12 DNA without the motifs were isolated from E. coli GM2163 and used to transform the indicated strains. Transformation frequency was calculated as number of transformants relative to the total amount of cells in transformation mixtures. Mean frequency for PAO1161 cells transformed with pCM132 was set to 100%. Lines indicate means and dots indicate results of independent transformations. b Influence of the source of plasmid DNA on its ability to transform P. aeruginosa strains. pCM132 isolated from the indicated P. aeruginosa strains was used for transformation. The experiment was performed twice with identical results. (+) at least 50 colonies on the plates, (−) no colonies

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BMC Genomics

ISSN: 1471-2164

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