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Fig. 1 | BMC Genomics

Fig. 1

From: Exploring the changing landscape of cell-to-cell variation after CTCF knockdown via single cell RNA-seq

Fig. 1

Knockdown of CTCF and schema of single cell sequencing. a. Western blot analysis of CTCF in luciferase control (shLuc) and CTCF-KD cells (shCTCF#1 and shCTCF#2). EL4 cells were infected with retroviral particles encoding GFP and an shRNA targeting CTCF or a control sequence for 5 days. b. Real-time quantitative PCR (RT-qPCR) analysis of CTCF expression in luciferase control (shLuc) and knockdown (shCTCF#1 and shCTCF#2) cells. The expression level of CTCF was normalized to GAPDH. c. Schema of single cell RNA sequencing using Fluidigm C1 system

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