Fig. 5

Relative expression and functional distribution of shared DEGs with DE AS under ABM and TXM treatments. a Venn diagram represents shared and unique DEGs and DE AS under ABM and TXM treatments. b Heat map represents the expression level of selected genes along with their functions and AS activity under insecticides treatments. Transcript levels following insecticides treatments are depicted using FPKM values on a color scale. The spots highlighted in Pink-magenta indicated the DEGs exhibit a significant expression level compared with control after treatments. c Expression level of the representative DE AS gene Os03g12620 under control, ABM and TXM treatments. The left side figure represents the AS score, while right side shows the expression level of AS-mediated gene depicting negative relationship. d Enriched MapMan pathways for DE AS events expressed under control, ABM and TXM treatments. The y-axis represents the distribution of genes into different cellular components, and x-axis shows gene numbers indicated in front of each bar. e An example of Exon skipping (ES) of Os06g39344 gene along with its AS score (lnc level) at 1d treatment under control or ABM treatments. Predicted graphical representation of the AS activity can be observed in the form of one exon skipping from the ABM treated samples. f Expression profile of representative DE AS gene under control, ABM and TXM treatments at three different intervals. The left side graph represents the AS score of DE AS, while right side shows the expression level of the gene with negative correlation reducing the expression level of the gene under pesticides treatments compared to control samples