Fig. 6

Relative expression of representative genes at further time points. Expression levels in susceptible (red bars) and resistant (green bars) fish at different days were measured using RT-qPCR, normalized to those of ef1a and the fold changes were calculated relative to the mean of one resistant family at day 0. Each panel refers to a different gene that was initially found to be differentially expressed between day 0 and 4 in susceptible or resistant fish in the RNA-Seq analysis. a and b interferon induced genes. Both vip2 (a) and mxe (b) were up-regulated only in susceptible fish between day 0 and 4 based on the RNA-Seq data. c to e chemokine genes. Comparing the RNA-Seq data between days 0 and 4 identified ccl19a1.1 (c) was up-regulated only in susceptible fish, while cxcl8b1.2 (d) and cxcl8b1.3 (e) were up-regulated only in resistant fish. None of the within-day comparisons between resistant and susceptible fish were statistically significant. In all panels, changes in expression that were significantly different from day 0 within the same fish type are marked by asterisks (*)