Venomics of the ectoparasitoid wasp Bracon nigricans

BMC Genomics

Table 1 Overview of the de novo transcriptome assembly of Bracon nigricans venom glands

Sequencing and assembly parameters	Value
Total number of raw reads	25,252,591
Paired-end reads after cleaning	24,437,756
Single reads after cleaning	796,318
Collapsed transcripts/unigenes¹	42,334/25782
N₅₀ of unigenes (bp)²	2636
% Reads mapped	93.3
Transcripts vs Bracon nigricans venom (proteins)³	4783 (109)
Transcripts vs UniprotKb (proteins)³	22,218 (9915)

¹The number of transcripts/unigenes resulting after the assembly by Trinity and the subsequent collapsing step by CD-HIT
²N₅₀ value, which represent the threshold delimiting 50% of the contigs in the entire assembly which are equal to or larger than the reported value
³e-value = 1e-5, in brackets the number of unique proteins found

ISSN: 1471-2164