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Table 2 Statistical comparison of transcriptome assembled by different sequencing platforms

From: Full-length transcriptome sequences of Agropyron cristatum facilitate the prediction of putative genes for thousand-grain weight in a wheat-A. cristatum translocation line

 

Proportion of non-existing transcriptsc

Proportion of unmapped readsd

BUSCO analysis with fragment ratioe

N50 (bp)

Illumina_1a

7.35%

18.28%

31.10%

1026

Illumina_2b

23.3%

48.02%

8.80%

651

PacBio

13.67%

72.24%

6.30%

3572

  1. Notes: a represents transcripts assembled by Zhang [20] using the Illumina GAII sequencing platform; b represents transcripts assembled by Zhou [20] using the Illumina HiSeq 2500 sequencing platform; c represents the proportion of transcripts with zero coverage after realignment of reads on the transcriptome; d represents the proportion of unmapped reads that were not mapped to the transcriptome; e represents the proportion of incomplete transcripts in the BUSCO analysis