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Fig. 4 | BMC Genomics

Fig. 4

From: Overexpression of a modified eIF4E regulates potato virus Y resistance at the transcriptional level in potato

Fig. 4

Potato virus Y levels in the ATL07 and ATLWT plants. a Boxplot showing the transcript per million (TPM) of PVYO and PVYN:O with respect to the S. tuberosum reference transcriptome in the ATL07 and ATLWT plants following mock- and/or PVY-inoculation. Each box is represented by three repetitions with three technical replicates each. Letters represent groups that showed significant mean TPM differences using Tukey’s Honestly Significant Difference (HSD) Test (P-value < 0.001). b Neighbor-Joining tree showing the phylogenetic affinity of the PVY assemblies from the PVY-inoculated WT plants. PVY genomes were assembled with NCBI Magic-BLAST RNAseq mapping tool using the reference PVY genome (NC_001616) as mapping template. Assemblies and consensus sequences were analyzed using IGV [34]. c Comparison of the amplification speeds in the RT-LAMP assay for PVY coat protein detection from total RNA isolated from ATL07 and ATLWT plants following mock- or PVY-inoculation. We used no template as a negative control. As a positive control, we included total RNA from PVYO and PVYN:O inoculum sources

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