Fig. 1

Prediction of 80 secondary metabolite biosynthesis clusters in the genome of Sclerotinia sclerotiorum. The left circular plot shows chromosomes 1 to 8 and the right one shows chromosomes 9 to 16. Chromosome numbers and genomic coordinates in kilobases (KB) are labelled around the peripheries of the plots. The outer-most track depicts expression data from the time course published in Seifbarghi et al. (2017) [4]. From bottom to top, the samples are 1, 3, 6, 12, 24 and 48 h post inoculation (HPI) of detached Brassica napus leaves. Expression data are plotted as log (fold change) relative to expression during growth in minimal medium. Log (fold change) goes from green (low) to zero (black) to red (high). The next track (‘Final’) shows the genomic coordinates of the final 80 secondary metabolite biosynthetic gene clusters (BGCs) predicted in the Sclerotinia sclerotiorum genome. The coloured lines emanating towards the heat map join each of the genes in the clusters to a representation of its time course expression data. The black lines represent genes that exhibited significant coexpression with their neighbours; green lines represent those that did not. The next track (‘aSMASH’), in blue, shows the positions of AntiSMASH secondary metabolite cluster predictions. The final track (‘SMURF’), in dark red, shows the positions of SMURF secondary metabolite BGC predictions. The final gene clusters depicted in track two were based on manual curation and merging of these two outputs