Fig. 3

Comparison between Piphillin and PICRUSt2 using DADA2-corrected ASV data. a Spearman’s correlation coefficient against corresponding shotgun metagenomics results were compared for two datasets. Spearman’s correlation coefficient was calculated for each sample and ranges are depicted as box and whisker plots as described in Fig. 1. b Comparison of log2FC in differential abundance analysis of KOs between metagenomic and Piphillin-predicted data. Color based on comparison to metagenomics results, in which the adjusted p-value cutoff was 0.2 for significance. c Comparison of log2FC in differential abundance analysis of KOs between metagenomic and PICRUSt2-predicted data. Color based on metagenomics results, in which the adjusted p-value cutoff was 0.2 for significance. d False positive rate, true positive rate/recall, balanced accuracy, and precision of detecting significant differences between cancer and healthy human oral biopsy samples were compared. * indicates p < 0.05, ** indicates p < 0.001, *** indicates p < 0.0001