Fig. 3

Conservation of Brassica oleracea genes across the four re-sequenced genomes of Brassica cretica. The Venn diagram shows the numbers of genes confirmed to be present in each of the four genomes. Genes and reference genome sequence were taken from the Bolbase database [31]. Sequence reads were aligned against the reference genome with BWA and coverage of each gene calculated using the coverageBed utility in BEDtools [32]. A gene was considered present only if it was 100% covered by sequence reads. The Venn diagram was rendered using the webserver [33]. The full list of coverages for each gene is provided in Additional File 3: Table S5