Fig. 1

Fusarium circinatum inoculation of Pinus pinaster. a-c: symptoms at the shoot tip of inoculated Pinus pinaster seedlings at each sampling time point. a: 3 dpi, no visible symptoms. b: 5 dpi, no visible symptoms. c: 10 dpi, symptoms at the inoculation site. e, g, i: transverse sections of P. pinaster inoculated shoot tip visualized under epifluorescence microscope at each sampling time after inoculation. d, f, h: growth of the fungus in the resin drop at each time point. d-e: 3 dpi. f-g: 5 dpi. h-i: 10 dpi. White arrows indicate colonization of the fungus within host tissue. Bar 100 μm. j: progression of a green fluorescent protein (GFP)-tagged Fusarium circinatum strain within Pinus pinaster shoot tissue at different days after inoculation