Fig. 5

Characterization of a two-component lanthipeptide from S. rimosus.a The biosynthetic gene cluster of birimositide and the predicted structures of Brtα and Brtβ with the cyclization pattern in comparison to those of lacticin 3147 (Ltnα and Ltnβ). The location of modified residues in Brtα and Brtβ are based on tandem mass spectrometry data (Supplementary Figure S13) and homology with lacticin 3147. Ser and Thr derived residues are colored in red as well as Ser and Thr, whereas Cys derived residues are colored in blue. Dha: dehydroalanine, Dhb: dehydrobutyrine, 2-ob: 2-oxo-butyrate moiety derived from Dhb. b) MALDI-TOF mass spectra showing Brtα and Brtβ in crude extract of S. rimosus culture, as well as purified Brtα and Brtβ. The masses correspond to the predicted core peptides dehydrated 7-fold (Brtα) and 6-fold (Brtβ) and the addition of hydrogen for the reduction of Brtβ