Fig. 3

Comparison of unique or conserved TSGs in the pig using cross-species analysis. a Clustering of samples based on expression values, FPKM of singleton orthologous genes present in all five species (n = 10,740) are calculated. Single linkage hierarchical clustering is used. (Bottom right) Phylogenomic relationships of the five mammals. b Factorial map of the principal component analysis of expression levels for 1:1 orthologous gene. The proportion of variance explained by the principal components is indicated in parentheses. c Bar charts represent the number of all TSGs (All) and single-copy TSGs (SC) in each mammal. d Number of unique TSGs and conserved TSGs in the pig. The 10,740 1:1 orthologous gene identified are used as a reference. e-f Comparison of expression levels in testis (e) and tissue specificity index scores (f) between LCTSGs (n = 87), MCTSGs (n = 113) and HCTSGs (n = 195), respectively. The statistical test in the panel is based on the one-sided Wilcoxon rank-sum test. * P < 0.05; ** P < 0.01; *** P < 0.001. g Functional annotation of the three gene sets (LCTSGs, MCTSGs and HCTSGs) in the pig