Fig. 8
Validation of the expression levels of porcine TSGs using qRT-PCR. Histograms show mRNA expression levels of eight TSGs randomly selected in different tissues (including testis, brain, heart, liver, kidney and muscle) of LY pig (n = 3). The blue line represents the results of the RNA-seq analysis (expression levels: log10(FPKM+ 1) transformed). Ct values above 35.0 are considered not biologically significant in this study, and tissue values are not shown in these cases. Data represent Mean ± SEM. * P < 0.05; Values with different letters (a-c) differ significantly at P < 0.05