Fig. 6

Chi-miR-30b-5p directly targets CaMKIIδ gene. (a) A Venn diagram depicting the overlap of the results of chi-miR-30b-5p targets predicted by Targetscan databases with different expressed genes (p-value < 0.005, were included in the analysis) related with HF development in anagen and telogen. (b) 17 DEGs were shown in Heat Map. (c) Sequence of chi-miR-30b-5p and its predicted binding site in FZD3–3’UTR, CaMKIIδ-3’UTR and FRS2–3’UTR. (d) Dual luciferase assay to detect the interaction between chi-miR-30b-5p and CaMKIIδ-3’UTR, after co-transfecting the corresponding report vectors with miR-30b-5p mimic or inhibitor in HEK293T. (e) The dual luciferase assay with chi-miR-30b-5p and FZD3–3’UTR. (f) The dual luciferase assay with chi-miR-30b-5p and FRS2–3’UTR. The data were presented as mean ± SEM for 3 biological replicates, *P < 0.05