Fig. 3

. Variant interference in 10 de novo assemblers. a Schematic diagram depicting concepts of the VD, VI, and VS, and their relationship to PID. b Comparison of output from 10 different assemblers. The number of contigs produced by each de novo assemblers at different variant PID ranges (75–99.6%) were shown. c Close-up of PID ranges where variant interference is the most apparent. Blue denotes de Bruijn graph assemblers (DBG); green denotes overlap-layout-consensus assemblers (OLC); orange denotes commercialized proprietary algorithms. Variant distinction, VD; variant interference, VI; variant singularity, VS. *For SOAPdenovo2, several data points returned zero contigs due to a well-documented segmentation fault error. The y-axis denotes the number of contigs