Fig. 6

The effect of variant interference on the assembly of four clinical datasets using three assembly programs. Fastq reads were partitioned into four categories: total reads (T), major variant (M), minor variant (m), and background (B). These reads were then combined into four different categories: T, M, major and minor variants (Mm), and major variant and background (MB). Datasets were assembled using SPAdes, Cap3, and Geneious. The bar graphs show the UG₅₀% metric and the length of the longest contig. Coxsackievirus B5, CV-B5; Enterovirus A71, EV-A71; Parechovirus A3 (Sample 1), PeV-A3 (S1); Parechovirus A3 (Sample 2), PeV-A3 (S2)