Skip to main content

Table 6 Optimized accD and ycf1 barcode primers for the family Asteraceae

From: Comparative chloroplast genome analysis of Artemisia (Asteraceae) in East Asia: insights into evolutionary divergence and phylogenomic implications

Barcode

Primer

Sequence (5′ to 3′)

accD-1 k

Forward

Consensus at 95% thresholda

CGATGTTATTTAAGAMGGAGTTCG

 

accD-Asteraceae-Fb

CGATGTTATTTAAGAAGGAGTTCG

Reverse

Consensus at 95% thresholda

CAAACGGGTAATTTTCTCCCC

 

accD-Asteraceae-Rb

CAAACGGGTAATTTTCTCCCC

Expected amplicon size (bp)

928–1079

ycf1b

Forward

Consensus at 95% thresholdd

RMTCGACGAAAATCYGGTTCTTCYAAAT

 

ycf1b-Asteraceae-Fc

GCTCGACGAAAATCCGGTTCTTCCAAAT

 

ycf1bFe

TCTCGACGAAAATCAGATTGTTGTGAAT

Reverse

Consensus at 95% thresholdd

WTACATGYSAAAGTGATGGAAAA

 

ycf1b-Asteraceae-Rc

ATACATGCCAAAGTGATGGAAAA

 

ycf1bRe

ATACATGTCAAAGTGATGGAAAA

Expected amplicon size (bp)

874

  1. aConsensus at 95% threshold across 212 accD sequences from the family Asteraceae
  2. b, cOptimized primers for the family Asteraceae
  3. dConsensus at 95% threshold across 211 ycf1 sequences from the family Asteraceae
  4. eUniversal barcode primer set designed for angiosperm [30]