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Fig. 6 | BMC Genomics

Fig. 6

From: Genome-wide profiling of host-encoded circular RNAs highlights their potential role during the Japanese encephalitis virus-induced neuroinflammatory response

Fig. 6

CircRNA_0000220 positively regulates the production of JEV-mediated inflammatory cytokines and acts as a ceRNA of miR-326-3p. a The relative expression level of circ_0000220 measured in CRISPR/Cas9 knockdown BV2 cells (circ_0000220_KD) or wild-type BV2 cells (NC). b Circ_0000220 knockdown BV2 cells or wild-type BV2 cells were infected with JEV at MOI of 5 MOI. At 24 h post-infection, samples were collected and mRNA expression levels of TNF-α, IL-6, IL-1β, and CCL5 were measured by quantitative real-time PCR. c and d The wild-type BV2 cells were transfected with miR-326-3p mimics or mimics control (miR-NC) for 24 h, followed by infection with JEV at 5 MOI. At 24 h post-infection, the relative mRNA expression levels of miR-326-3p (c) and TNF-α, IL-6, IL-1β, and CCL5 (d) were measured by quantitative real-time PCR. e The wild-type BV2 cells were transfected with miR-326-3p mimics or mimics control (miR-NC) for 24 h. The relative expression levels of circ_0000220 and mRNAs (BCL3, MK2, and TRIM25) were measured by quantitative real-time PCR. f HEK-293 T cells were transfected with miR-326-3p mimics or mimics control (miR-NC) together with luciferase reporter plasmid harboring the 3′ UTR of circ_0000220, BCL3, MK2, or TRIM25 for 24 h, and followed by measurement of the luciferase activity. Data are expressed as mean ± SEM from three independent experiments

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