Fig. 4
HDAC3 knockdown in the last instar larvae of T. castaneum affects the transcription of genes involved in multiple pathways. A Heatmap of RNA-seq data. The heatmap of normalized mean expression values of 741 differentially expressed genes (≥ 2- fold and a P < 0.05) between dsmalE and dsHDAC3 treated insects. B Differentially expressed genes identified after HDAC3 knockdown represented as the volcano plot. The X and Y-axis represent the -log10P- values and log2 fold change of mean normalized values, respectively. The red dots indicate the genes that showed ≥2- fold difference in expression with a P < 0.05. C RT-qPCR verified the expression of 20 selected genes from the up-regulated group (RNA-seq data). Descriptions of genes are listed in (Additional file 1, Table S4)