Fig. 5

Confirmation of HDAC3 targets in T. castaneum pupae and TcA cells. A 72 h-old last instar larvae were injected with dsHDAC3 or dsmalE. Total RNA was extracted at five days after injection, and mRNA levels were quantified. The mean ± SE of four replications is shown. The data were analyzed using analysis of variance, each pair student’s t-test. Mean values with the same letter are not significantly different from each other. TcA cells were treated with dsHDAC3 or dsmalE. Total RNA was extracted 72 h after dsRNA treatment, and mRNA levels were quantified. The mean ± SE of four replications is shown. The data were analyzed using analysis of variance, each pair student’s t-test. Mean values with the same letter are not significantly different from each other