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Fig. 6 | BMC Genomics

Fig. 6

From: Histone deacetylase 3 is required for development and metamorphosis in the red flour beetle, Tribolium castaneum

Fig. 6

HDAC3 knockdown affects acetylation levels of histone H3 in T. castaneum larvae. A The knockdown of HDAC1 and HDAC3 increased acetylation levels of histone H3. Total protein extracted from dsHDAC1, dsHDAC3 or dsmalE injected larvae were separated on SDS-PAGE gels, transferred to western blots, and hybridized with antibodies recognizing Acetyl-Histone H3 (Antibody Sampler Kit # 9927-Cell Signaling. ß-actin served as a loading control. The HRP-linked IgG (#7074-Cell Signaling) was used as a secondary antibody. The lysine acetylation status of histone H3K9 and H3K27 increased in HDAC3 knockdown larvae. The images of complete Western blots are shown in Additional file 5. B Loading control protein, ß-Actin, was used to normalize the levels of H3K9 and H3K27. Band intensities were determined by Image-J software. The fold change in treatments compared to control was represented in the graph

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