Fig. 5
Validation of upregulated and downregulated DEGs in IR64 indica rice. a Validation of upregulated and downregulated genes using qRT-PCR. Histogram showing expression profiles of 15 upregulated (red bars) and 10 downregulated (green bars) genes, under 2 h cold shock (at 4 °C) conditions. Filled bars correspond to qRT-PCR data, and striped bars represent RNA-seq data, respectively. Error bar represents mean ± S.D. (n = 3; Two-tailed paired t-test, the level of significance was represented by * (where *P < 0.05 and > 0.0332, **P < 0.0332 and > 0.0021, ***P < 0.0021 and > 0.0002, ****P < 0.0001). b Validation of upregulated genes of the DREB regulon using qRT-PCR. Histogram showing expression profiles of 20 genes. Error bar represents mean ± S.D. (n = 3; Two-tailed paired t-test, the level of significance was represented by * (where *P < 0.05 and > 0.0332, **P < 0.0332 and > 0.0021, ***P < 0.0021 and > 0.0002, ****P < 0.0001)