Fig. 4

Sequencing quality of TruDrop libraries on exAmp chemistry sequencers. a The base calling accuracy plot for two dual-indexed TruDrop libraries on iSeq alongside PhiX. Cycles 1–50 depict the quality scores for the cell barcode + UMI read. Cycles 51–151 are sequence data that will be trimmed and discarded during analysis. Cycles 152–159 correspond to the i7 index read. Cycles 160–167 are the i5 index read. Cycles 168–318 are the transcript read. For the purpose of direct comparison only cycles 168–267 are marked as transcript as only 100 bases of transcript were sequenced for the V2 libraries. b The base calling accuracy plot for the same two TruDrop libraries when sequenced on the NovaSeq alongside 107 other libraries. c The base calling accuracy plot for 24 dual-indexed TruDrop library sequenced on a NovaSeq alongside 186 other libraries