Fig. 4

PaHV-2 phylogeny and clades. A genome sequence alignment was generated with the available PaHV-2 strains (Table 1). A phylogenetic network (Figure a) was generated using the HKY + G + I substitution model recommended by IQ-Tree (gaps deleted; p-inv = 0.572; gamma = 0.739). Figure b shows a maximum likelihood tree which shows three clades. Pairwise distances between the strains were plotted (Figure c) and a clade cutoff value calculated (1.96%). Figure d includes a table showing the between group genetic distances