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Table 1 Generalized linear model comparing gene detection of the Freeze-thaw protocol to the other tested protocols. Comparison of the number of genes detected for Smart-seq2, and five modified Smart-seq2 variants, against our “Freeze-thaw” protocol using a generalized linear model with a negative binomial error distribution. Significant p-values (<= 0.05) are indicated with an asterisk

From: An efficient single-cell transcriptomics workflow for microbial eukaryotes benchmarked on Giardia intestinalis cells

FPKM

Protocol

Estimate

StdError

z-value

Pr(>|z|)

0

Smart-seq2

−0.045146

0.036829

−1.226

0.22026

0

Half volumes

−0.098322

0.036848

−2.668

0.00762*

0

5′ Biotin mod.

−0.035167

0.038118

−0.923

0.35622

0

In-house beads

−0.050162

0.03683

−1.362

0.17321

0

1 μM oligo-dT

−0,004165

0.036814

−0.113

0.90992

0

All changes

−0.022638

0.036821

−0.615

0.53867

0.1

Smart-seq2

−0.12486

0.06261

−1.994

0.0461*

0.1

Half volumes

−0.14494

0.06261

−2.315

0.0206*

0.1

5′ Biotin mod.

−0.07958

0.0648

−1.228

0.2194

0.1

In-house beads

−0.10104

0.0626

−1.614

0.1065

0.1

1 μM oligo-dT

−0.07918

0.0626

− 1.265

0.2059

0.1

All changes

−0.0756

0.0626

−1.208

0.2271

1

Smart-seq2

−0.26975

0.12333

−2.187

0.0287*

1

Half volumes

−0.11903

0.12331

−0.965

0.3344

1

5′ Biotin mod.

−0.23547

0.12766

−1.845

0.0651

1

In-house beads

−0.13111

0.12331

−1.063

0.2876

1

1 μM oligo-dT

−0.20511

0.12332

−1.663

0.0963

1

All changes

−0.20074

0.12332

−1.628

0.1036