Fig. 7
From: RNA-Seq of three free-living flatworm species suggests rapid evolution of reproduction-related genes
Whole-mount in situ hybridisations for OG0014441. Expression of the transcript affecting the sperm bristle phenotype and its orthologs in the testes of in M. lignano (a), M. hystrix (b), M. spirale (c), and M. pusillum (d). Unspecific staining (asterisks) can be seen in pharyngeal glands and in the tail region (staining of pharyngeal glands is also present in control experiments with the sense probe; see also Additional file 12: Fig. S2). Transcripts RNA815_7008 (M. lignano), Machtx_20180703@G07456_i1 (M. hystrix), Macspi_20180703@G161928_i1 (M. spirale), and Macpus_20180703@G35224_i1 (M. pusillum) of orthogroup OG0014441 were used for in situ probe generation. Scale bars: 100 μm