Table 1 Transcriptome assembly statistics per species. The initial number of reads used, the number of reads after Trimmomatic processing, the number of initially assembled transcripts, the empirical mean insert size of the RNA-Seq libraries, the number of distinct 21-mers, the number of transcripts removed by CroCo, and the final number of transcripts, as well as the mean transcript length and number of bases in the final assemblies are shown. The BUSCO score is given as the percentage of complete (C) genes—divided into present as single copies (S) or duplicates (D)—and fragmented (F) genes of the 978 metazoa gene set. The next three rows detail the TransRate score, the number of transcripts remaining after TransDecoder translation and CD-HIT clustering, and the number of transcripts considered in the DE analysis. Below this a summary of the results from the Trinotate annotations giving the number of transcripts (and the corresponding percentage of the whole transcriptome in brackets) with a given annotation: ORF, contains a predicted open reading frame; BLASTX, the predicted ORF and/or the entire transcript produced a hit in the protein database; Pfam, a protein family domain was found; SignalP, a signal peptide was detected; TMHMM, a transmembrane helix is predicted

From: RNA-Seq of three free-living flatworm species suggests rapid evolution of reproduction-related genes