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Fig. 8 | BMC Genomics

Fig. 8

From: Decoding the differentiation of mesenchymal stem cells into mesangial cells at the transcriptomic level

Fig. 8

Experimental design of the study. a MSC were co-cultured with injured mesangial cells. On indicated days, co-cultured MSC were harvested and RNA was extracted. cDNA was synthesised and subsequently underwent RNA-seq. b After RNA-seq, raw data was processed by initial read processing and normalizations before the data were further analyzed simultaneously using two methods. c Key genes related to MSC or mesangial cells with ascending or descending monotonic patterns over the differentiation process were identified with MFSelector. d Co-expression patterns between TF and construction TF co-expression network were determined by TO-GCN. Once the data from both methods was generated, both sets of data were analysed synergistically (e). The data obtained from both methods were used to correlate the key genes at specific time points to the TO-GCN at different levels. This helps to elucidate the network interaction between TF-TF and TF-key genes at each level of TO-GCN. Additionally, this builds our understanding of the overall gene expression during the MSC differentiation into mesangial cells

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